PCR-SSCP Polymorphism of Inhibin ??A Gene in Some Sheep Breeds
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چکیده
منابع مشابه
Association of the Polymorphism Kiss1 Gene Exon 1 with Twin Traits in Sanjabi, Ghezel Breed Sheep by PCR–SSCP Technique
The Kiss1 gene is one of the known affective gene in fertility, which affects ovulation and multiplication. In this study, Blood samples were collected from 100 sheep of Sanjabi in Mehregan breeding station in Kermanshah province and 50 sheep Ghezel breeding station of Urmia University. After DNA extraction, the polymerase chain reaction (PCR) was used for specific primers amplification of 331 ...
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The present investigation was to study DNA polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene by PCR-RFLP in four Egyptian local sheep breeds. Genomic DNA was isolated from a total of 20 animals from four Egyptian breeds (five each) of sheep namely Rahmani, Ossimi, Awassi, and Barki. A fragment of IGFBP-3 gene, comprising of a part of exon 2, complete intron 2, exon 3, ...
متن کاملPolymorphism of some Genes Associated with Meat-Related Traits in Egyptian Sheep Breeds
The genetic polymorphism of some genes related to meat production in three Egyptian sheep breeds (Barki, Rahmani and Osseimi) was studied. The candidate genes were: Calpastatin, Myostatin, Diacylglycerol-acyltransferase1, Insulin-like growth factor binding protein-3 and Booroola fecundity gene. The technique applied was the restriction fragment length polymorphism for the polymerase chain react...
متن کاملAssociation of Exon 4 Region of Gpr54 Gene Polymorphisms with Litter Size Trait in Iranian Sanjabi and Ghezel Sheep Breeds by PCR –SSCP
G-protein-coupled receptor (GPR54) is one of the known gene affecting fecundity. This gene affects the ovulation rate and litter size performance. A total of 160 animals including Sanjabi (n=100) and Ghezel (n=60) were used to identify polymorphisms of GPR54 gene and their influence on litter size. Genomic DNA was extracted by commercial DNA kit. The genotypic patterns were detected using th...
متن کاملdetection of polymorphism in the exon 4 and 3ʹ utr of tnfα gene in makoei sheep, using pcr-sscp
tumor necrosis factor -alpha (tnfα) is a proinflammatory cytokine that plays critical roles in the regulation of immune responses including macrophages activity and apoptosis. tnfα gene is polymorphic and its haplotypes have been associated with resistance or susceptibility to disease. this study was undertaken to identify the polymorphism of tnfα gene in makoei sheep, using polymerase chain re...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2007
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2007.1023